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PCR SuperMix
  • AS122-01.JPG
AS122-01.JPG

TransTaq®-T PCR SuperMix (-dye)


产品介绍

本产品含TransTaq®-T DNA Polymerase、dNTPs和优化的反应缓冲液,浓度为。DNA扩增时,只需加入模板,引物和水,使SuperMix溶液的浓度为即可进行反应。其扩增产物3′端带“A”碱基(加“A”效率和EasyTaq® DNA Polymerase相同),进行PCR增可以减少操作时间,避免因多步操作带来的污染。具有高保真(保真性和EasyPfuDNA Polymerase相当),高扩增效率等优点。TransTaq® -T PCR SuperMix(-dye)扩增产物可直接克隆于pEASY® -T系列 载体中。TransTaq®-T PCR SuperMix (+dye)扩增产物可直接点样电泳,如用于克隆时,需纯化去掉染料。 
• 高加"A"效率,可直接克隆于pEASY®-T载体中,克隆效率高。

• 保真性和EasyPfu DNA Polymerase相同,是EasyTaq® DNA Polymerase18倍。
• 基因组DNA 片段的扩增(≤8 kb)。

References

Li D M , Hou Y , Song X P ,et al. High Prevalence and Genetic Heterogeneity of Rodent-Borne Bartonella Species on Heixiazi Island, China[J]. Applied and Environmental Microbiology, 2015, 81(23):7981-7992.

Wang J , Lin P , Zhao H ,et al. Continuing evolution of canine parvovirus in China: Isolation of novel variants with an Ala5Gly mutation in the VP2 protein.[J]. Infection Genetics&Evolution, 2016, 38:73-78.

Xiang R H , Juan Y , Peng G ,et al. Bartonella Species Detected in the Plateau Pikas (Ochotona curzoiae) from Qinghai Plateau in China[J]. Biomedical and Environmental Sciences, 2015, 28(9):674-678.

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