RT-PCR
  • AH341-01.JPG
AH341-01.JPG

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)


产品介绍

本产品含有反转录反应所需的全部试剂(TransScript® II RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer),浓度为。反应时,只需加入gDNA Remover、模板RNA和水,在42℃-55℃条件下即可高效地合成第一链cDNA,同时去除RNA模板中残留的基因组DNA。另配有TransScript® II All-in-One No-RT Control SuperMix for qPCR,用于配制无反转录酶的对照,判断qPCR模板是否来自cDNA。该产品操作简便,降低操作过程中的污染机率。cDNA只适用于qPCR,不适用于常规PCR

产品组成

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使用前请将各组分点甩离心

References

Cui X . Exploring the formation and recognition of an important G-quadruplex in a HIF1α promoter and its transcriptional inhibition by a benzo[c]phenanthridine derivative.[J]. Journal of the American Chemical Society, 2014, 136(6):2583.

Gu Q , Chen Z , Yu X , et al. Melatonin confers plant tolerance against cadmium stress via the decrease of cadmium accumulation and reestablishment of microRNA-mediated redox homeostasis[J]. Plant Science, 2017, 261:28-37.

Huang X , Huang B , Chen J , et al. Cellular responses of the dinoflagellate\r, Prorocentrum donghaiense\r, Lu to phosphate limitation and chronological ageing[J]. Journal of Plankton Research, 2016, 38(1):83-93.

Guo J , Zu G , Zhou T , et al. Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer[J]. Clinical and Translational Oncology, 2015, 17(10):788-794.

FAQ 产品指南

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