点突变
  • FM111-01.JPG
FM111-01.JPG

Fast Mutagenesis System


产品介绍

计原理:

(1) 引物与模板链退火,2xTransStart® FastPfu Fly PCR SuperMix合成突变链;(2) DMT酶消化体外降解非突变型质粒模板(甲基化质粒模板)和DMT感受态细胞体内降解非突变型质粒模板(甲基化质粒模板),从而有效地筛选突变克隆。


产品组成

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References

Wang J, Zhou L, Shi H, et al. A single transcription factor promotes both yield and immunity in rice[J].Science, 2018, 7, 361(6406):1026-1028.
Li W , Zhu Z , Chern M , et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance[J]. Cell, 2017, 170(1):114-126.e15.
Zhou X, Wang B, Pan Q, et al. Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history[J]. Nature Genetics, 2014, 46(12):1303-1310.
Zhang H , Lin X , Han Z , et al. Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs[J]. Cell Research, 2016, 26(5):543-55.
Song N , Liu Z S , Xue W , et al. NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation[J]. Molecular Cell, 2017, 68(1):185-197.

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